Virology I: How Viruses Work

Week 1
Welcome to virology
What is a virus?
Viruses then and now
Week 2
The infectious cycle
Assay of viral infectivity
Measurement of virions and their components
Revolutionary methods
One-step growth cycle
Week 3
The Baltimore scheme
DNA virus genomes
RNA virus genomes
Viral genetics Week 4
Structure
The tools of structural virology
Helical symmetry
Icosahedral symmetry
Enveloped virions
Complex virions Week 5
Attachment to cells
Entry into cells
Acid-catalyzed fusion
A new paradigm for entry
Entry of non-enveloped virions
Entering the nucleus Week 6
Viral RNA synthesis
RNA polymerization
Plus strand RNA synthesis
Negative strand RNA synthesis
RNA synthesis of dsRNA genomes
RNA synthesis as a source of diversity Week 7
DNA basics
Lessons from SV40
Priming via DNA or protein
Viral origins
Big DNA viruses
Regulation of DNA synthesis Week 8
Transcription
Initiation of transcription
Viral transcription regulation
Capping and polyadenylation
Pre-mRNA splicing Week 9
Reverse transcriptase
Retroviruses
Reverse transcription
Integration
The provirus
Hepatitis B virus Week 10
End-dependent initiation of protein synthesis
Other decoding mechanisms
One mRNA, one protein?
Maximizing coding capacity of the viral genome
Regulation of translation: eIF2alpha
How viruses regulate cell translation
MicroRNAs Week 11
Principles of virion assembly
Getting to the right place
Making sub-assemblies
Concerted assembly: Budding
Genome packaging
Acquisition of an envelope and egress